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德国拜发(R-Biopharm)试剂 硕腾ZOETIS动物诊断试剂

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绵羊奶中山羊奶检测试剂盒
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产品: 浏览次数:1802绵羊奶中山羊奶检测试剂盒 
品牌: R-biopharm(德国拜发)
产品型号: R4802
产品规格: 48孔
最小起订量: 1 个
供货总量:
发货期限: 自买家付款之日起 1 天内发货
有效期至: 长期有效
最后更新: 2020-06-11 15:23
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详细信息

RIDASCREEN® GIS
Enzyme immunoassay for the detection of
goat's milk in sheep's milk
Art. No.: R4802
In vitro Test
Storage at 2 - 8 °C
Brief information
The RIDASCREEN® GIS (Art. No.: R4802) is an enzyme immunoassay for the
detection of goat's milk in sheep's milk.
All reagents required for the enzyme immunoassay - including standards - are
contained in the test kit.
The test kit is sufficient for 48 determinations (including standards).
A microtiter plate spectrophotometer is required for quantification.
Sample preparation: dilution
Time requirement: sample preparation (for 10 samples) ...approx. 5 min
test implementation (incubation time) .......1 h 30 min
Detection limit: 1 % goat's milk in sheep's milk
1. Intended use
The RIDASCREEN® GIS (Art. No.: R4802) is an enzyme immunoassay for the
detection of goat's milk in sheep's milk.
2. General
Sheep's milk is much more expensive than goat's milk, which is often used to
extend sheep's milk. Falsification of milk can be detected chromatographically or
by electrophoresis. These methods are laborious and require sophisticated
equipment. Therefore, they are of limited value in routine screening of milk. Using
the RIDASCREEN® GIS test, admixtures of goat's milk can be detected with a
detection limit of 1 % in sheep's milk with just little sample preparation.
3. Test principle
The basis of the test is the antigen-antibody reaction. The presence of goat's milk
in a sample is determined by the immunological detection of caprine IgG, which is
a natural constituent of goat's milk. The wells of the microtiter strips are coated
with specific antibodies against caprine IgG antibodies. Standards or the sample
solutions are added. In the case of tainted products, the antibodies contained in
the goat's milk will bind to the immobilized antibodies. Any unbound components
are removed in a washing step. After adding the conjugate (antibody peroxidaseconjugate
directed against caprine IgG) the enzyme conjugate will bind to the
caprine antibodies. Any unbound conjugate is then removed in a washing step.
Enzyme substrate (urea peroxide) and chromogen (tetramethylbenzidine) are
added to the wells and incubated. Bound enzyme conjugate converts the colorless
chromogen into a blue product. The addition of the stop solution (sulfuric acid)
leads to a color change from blue to yellow. The measurement is made
photometrically at 450 nm.
4. Reagents provided
Each kit contains sufficient materials for 48 measurements (including standard
analyses). Each test kit contains:
1 x Microtiter plate with 48 wells (6 strips with 8 removable wells each)
coated with specific antibodies against caprine IgG
6 x Standard (1.3 ml each) *)
Standard 1: 0 μg/ml goat IgG
Standard 2: 0.0625 μg/ml goat IgG (approx. 0.005 % goat’s milk)
Standard 3: 0.125 μg/ml goat IgG (approx. 0.01 % goat’s milk)
Standard 4: 0.25 μg/ml goat IgG (approx. 0.02 % goat’s milk)
Standard 5: 0.5 μg/ml goat IgG (approx. 0.04 % goat’s milk)
Standard 6: 1 μg/ml goat IgG (approx. 0.08 % goat’s milk)
1 x Conjugate (130 μl)...............................................................................red cap
peroxidase conjugated antibodies against caprine IgG
concentrate (100 x)
1 x Substrate (7 ml solution)..................................................................green cap
contains urea peroxide
1 x Chromogen (7 ml) ............................................................................. blue cap
contains tetramethylbenzidine
1 x Stop solution (14 ml) ......................................................................yellow cap
contains 1 N sulfuric acid
1 x Buffer (14 ml), ready to use
conjugate dilution buffer
*) The standards are already diluted 1:200 and the concentration given on the
labels of the standards are the diluted concentrations.
5. Materials required but not provided
5.1. Equipment:
− Microtiter plate spectrophotometer (450 nm)
− 10 μl-micropipette
− variable 20 μl - 200 μl- and 200 - 1000 μl-micropipettes
6. Warnings and precautions for the users
The stop solution contains 1 N sulfuric acid (R36/38, S2-26).
7. Storage instructions
Store the kit at 2 - 8 °C (35 - 46 °F). Do not freeze any test kit components.
Return any unused microwells to their original foil bag and reseal them together
with the desiccant provided at 2 - 8 °C (35 - 46 °F).
The colorless chromogen is light sensitive, therefore, avoid exposure to direct
light.
No quality guarantee is accepted after the expiration date on the kit label.
Do not interchange individual reagents between kits of different lot numbers.
8. Indication of deterioration of reagents
− Any bluish coloration of the the chromogen solution prior to test implementation
− A value of less than 0.7 absorbance units (A450 nm < 0.7) for the standard 6
9. Preparation of Samples
The samples should be stored in a cool place.
9.1. Milk samples
− dilute the milk samples 1:200 with distilled water (e.g. 10 μl milk sample + 2 ml
distilled water)
− use 100 μl of the diluted sample per well in the assay
10. Test implementation
10.1. Preliminary comments
Bring all reagents to room temperature (20 - 25 °C / 68 - 77 °F) before use.
The antibody enzyme conjugate (bottle with red cap) is provided as a
concentrate. Since the diluted enzyme conjugate has a limited stability, only the
amount which actually is needed should be reconstituted. Before pipetting, the
enzyme conjugate should be shaken carefully. For reconstitution, the conjugate
concentrate is diluted 1:100 in buffer; e.g. 10 μl conjugate concentrate +
1 ml buffer, sufficient for 1 microtiter strips.
10.2. Test procedure
Carefully follow the recommended washing procedure. Do not allow microwells to
dry between working steps.
1. Insert a sufficient number of wells into the microwell holder for all standards
and samples to be run in duplicate. Record standard and sample positions.
2. Add 100 μl of each standard solution or prepared sample to separate
duplicate wells and incubate for 30 min at room temperature (20 - 25 °C / 68 -
77 °F).
3. Pour the liquid out of the wells and tap the microwell holder upside down
vigorously (three times in a row) against absorbent paper to ensure complete
removal of liquid from the wells. Fill all the wells with 250 μl distilled water and
pour out the liquid again. Repeat two more times.
4. Add 100 μl of the diluted enzyme conjugate (see 10.1.) to each well and
incubate for 30 min at room temperature (20 - 25 °C / 68 - 77 °F).
5. Pour the liquid out of the wells and tap the microwell holder upside down
vigorously (three times in a row) against absorbent paper to ensure complete
removal of liquid from the wells. Fill all the wells with 250 μl distilled water and
pour out the liquid again. Repeat two more times.
6. Add 50 μl of substrate and 50 μl of chromogen to each well. Mix gently by
shaking the plate manually and incubate for 30 min at room temperature
(20 - 25 °C / 68 - 77 °F) in the dark.
7. Add 100 μl of the stop solution to each well. Mix gently by shaking the plate
manually and measure the absorbance at 450 nm. Read within 30 minutes
after addition of stop solution.
11. Results
A special software, the RIDA®SOFT Win (Art. No. Z9999), is available to evaluate
the RIDASCREEN® ELISA assays. Please use the software version 1.49 or
higher!
The course of the standard curve is shown in the joined Quality Assurance
Certificate.
The values calculated for the standards are entered in a system of coordinates on
semilogarithmic graph paper against the goat’s milk concentration in %.
The goat's milk concentration in % (0, 1, 2, 4, 8, 16 % adulteration) corresponding
to the absorption of each sample can be read directly from the calibration curve.
Remark:
The samples are also diluted 1:200 (see 9.1.) as the standards, therefore the
adulterations in % are as follows, because the dilution factor is already
taken into account:
standard 1: 0 %
standard 2: (0.005 x 200) 1 %
standard 3: (0.01 x 200) 2 %
standard 4: (0.02 x 200) 4 %
standard 5: (0.04 x 200) 8 %
standard 6: (0.08 x 200) 16 %
If other dilutions are requested, standards have to be prepared from freshly
spiked goat's milk using appropriate dilutions.
Due to great variability among goat's races for IgG concentration (alpine,
zannen etc.), you should prepare your own standard solutions from fresh
goat's and sheep's milk for better accuracy.
R-Biopharm makes no warranty of any kind, either expressed or implied,
except that the materials from which its products are made are of standard
quality. If any materials are defective, R-Biopharm will provide a replacement
product. There is no warranty of merchantability of this product, or of the
fitness of the product for any purpose. R-Biopharm shall not be liable for any
damages, including special or consequential damage, or expense arising
directly or indirectly from the use of this product.

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